Coding

Part:BBa_K1085001

Designed by: Claudio Tiecher   Group: iGEM13_Groningen   (2013-09-10)

RBS Start-codon SpiderSilkSubunitE1

This BioBrick contains the coding sequence for part of the MaSp2 spider silk protein (SubunitE1) with Bacillus subtilis ribosome binding site (RBS) in front of it.

The RBS is there to allow the ribosomes to bind and start to translate the DNA.

SubunitE1 codes for a spider silk protein that is optimized to prevent the forming of secondary RNA structures in close proximity to the RBS. This is given priority over reducing the number of restriction sites. However, the primary goal of the algorithm was still to optimize the codon selection based on their availability scores.

The amino acid sequence is based on the adapted MaSp2 sequences described in the paper of Brooks et. al. 2008.

Related BioBricks

BBa_K1085000 is the same except it has a Step-tag between the Start-codon and SilkSubunitE1.

BBa_K1085005 is the same except it has a SpiderSilkSubunitN1 instead of SpiderSilkSubunitE1.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/bsubtilis
//collections/silk
Parameters
None