Intermediate

Part:BBa_K1060007

Designed by: Ingmar Claes, Pieter De Bruyn, Sylvie Weckx   Group: iGEM13_KU_Leuven   (2013-09-17)

C0076:B0015

This part is an intermediate construct existing of the cinI gene BBa_C0076 followed by the double terminator BBa_B0015. cinI codes for an autoinducer synthetase and is derived from Rizobium etli. The quorum sensing molecule produced by this autoinducer synthetase is 3OH,C14:1-HSL.

This construct was made to be incorporated in an oscillator, that can be used for the alternating production of two different components. A quorum sensing molecule is necessary to get a colony-wide synchronization of oscillation. More information can be found on the KU Leuven 2013 wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None