Coding
EbfS Aa

Part:BBa_K1060002

Designed by: Ingmar Claes, Lukas Marcelis, Su Wang   Group: iGEM13_KU_Leuven   (2013-09-17)

EBF synthase from Artemisia annua

This open reading frame codes for a sesquiterpene synthase (GenBank Accession No. AY835398), (E)-β-farnesene synthase, that has been isolated from Artemisia annua L (Picaud et al., 2005). The enzyme converts farnesyl diphosphate into β-farnesene. β-farnesene is an alarm hormone produced by aphids. It is also an important semiochemicals in aphid localization. It is released in the cornicle secretions of many aphid species (Franscis et al., 2005) to alert surrounding aphids of the presence of natural enemies (Kunert et al., 2005). Detection of these short range chemical cues leads not to the aphid directly, but only indicates the presence, improving prey detection of the predators and parasitoids.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1306
    Illegal BamHI site found at 615
    Illegal XhoI site found at 577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None