Coding

Part:BBa_K1054011

Designed by: Jiasheng Wang   Group: iGEM13_ZJU-China   (2013-09-15)

BLF2 with linker

BLF2 is the C-terminal of ampicillin resistance gene TEM-1 β-lactamase. It consists of β-lactamase amino acid 198-290. It functions together with the C-terminal of β-lactamase (BLF1, BBa_K1054010), as when they dimerize they possess ampicillinase activity. [1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 131


[edit]
Categories
Parameters
None