Plasmid

Part:BBa_K090403

Designed by: Daniel Goodman   Group: iGEM08_Cambridge   (2008-10-29)

Gram-positive Shuttle Vector for Chromosomal Integration

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 5611
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5611
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5617
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5611
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 5611
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 5611
    Illegal XbaI site found at 5626
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1189
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3984
    Illegal EcoRI site found at 5611
    Illegal XbaI site found at 5626
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


[edit]
Categories
//plasmid/chromosomalintegration
//chassis/prokaryote/bsubtilis
Parameters
None