Reporter

Part:BBa_J58103

Designed by: Valencia iGEM 2006   Group: iGEM2006_Valencia   (2006-10-26)

Mutated promoter activated by OmpR-P with the reporter GFP

We have assembled promoter BBa_R0083 to GFP for characterization purposes. That promoter is regulated positively (activated) by the transcription factor OmpR-P (phosphorylated), and it contains only one operator in its sequence, whereas in the natural promoter BBa_R0082 there are three.

The deep components are as follows:
OmpR
R0083

B0034
GFP
E0040

B0010

B0012
This composite part will be very useful to characterize many cellular biosensors, jointly with the part BBa_J58102.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 748


[edit]
Categories
//classic/reporter/pret
Parameters
None