Mutated promoter activated by OmpR-P with the reporter GFP
We have assembled promoter BBa_R0083 to GFP for characterization purposes. That promoter is regulated positively (activated) by the transcription factor OmpR-P (phosphorylated), and it contains only one operator in its sequence, whereas in the natural promoter BBa_R0082 there are three.The deep components are as follows:BBa_J58102.
By Valencia iGEM 2006
A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).
1 Universidad Politecnica de Valencia, Spain
2 Universitat de Valencia, Spain
3 Ecole Polytechnique, France
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 748