Coding

Part:BBa_J52013

Designed by: Monika Ciglic   Group: iGEM2006_Slovenia   (2006-10-14)


dnMyD88-linker-Rluc-linker-PEST191

dnMyD88-linker-Rluc-linker-PEST191 is a part where dnMyD88 (very important protein in signaling cascade through TLR4 receptor) is linked to Rluc (reporter) and PEST191 (degradation tag). Both linkers are made of six aminoacids.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 982


[edit]
Categories
//cds/enzyme
//chassis/eukaryote/human
//function/immunology
//function/reporter/light
Parameters
None