Project
BOBS

Part:BBa_J45900

Designed by: Stephen Payne   Group: iGEM2006_MIT   (2008-02-04)

Banana odor biosynthetic system

We assembled a complete banana odor biosynthetic system (BBa_J45900) by combining the isoamyl alcohol (BBa_J45400) and banana odor generators (BBa_J45200). BBa_J45900 catalyzes the conversion of the cellular metabolite leucine to isoamyl acetate or banana odor.

BBa J45900.png

Usage and Biology

To construct a complete banana odor biosynthetic system, we needed to design an additional biosynthetic device for banana odor precursor production. We designed a second biosynthetic device for production of isoamyl alcohol from the cellular metabolite leucine (BBa_J45400) based on the BAT2 and THI3 genes from Saccharomyces cerevisiae. BAT2 encodes a cytosolic branched-chain amino acid transaminase that catalyzes the conversion of leucine to α-ketoisocaproate. THI3 encodes an α-ketoisocaproate decarboxylase that catalyzes the conversion of α-ketoisocaproate to 3-methylbutanal. 3-methylbutanal is reduced to isoamyl alcohol by an NADH-dependent reaction by an alcohol dehydrogenase. We anticipated that the final reduction step would be carried out by the cellular chassis. Indeed, cultures of the banana odor generator (BBa_J45200) supplemented with 3-methylbutanal produced banana odor.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal BglII site found at 3267
    Illegal BamHI site found at 4594
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
    Illegal AgeI site found at 1114
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3694
    Illegal SapI.rc site found at 4702


[edit]
Categories
//function/odor
s
Parameters
None