Plasmid_Backbone

Part:BBa_J428370

Designed by: Marcos Valenzuela-Ortega   Group: 2021_Engineering_Committee   (2022-06-15)


pJUMP46-2A SpecR Type IIS Level 2 vector. Origin p15A (medium copy number)

pJUMP46-2A SpecR Type IIS Level 2 vector. Origin p15A (medium copy number)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2339
    Illegal suffix found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2339
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 2345
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2339
    Illegal suffix found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2339
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2354
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1039
    Illegal NgoMIV site found at 1441
    Illegal AgeI site found at 1879
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2361
    Illegal BsaI.rc site found at 10


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/jump/level2
//plasmidbackbone/assembly/typeiis
Parameters
resistanceSpectinomycin