Plasmid_Backbone

Part:BBa_J428352

Designed by: Marcos Valenzuela-Ortega   Group: 2021_Engineering_Committee   (2022-06-15)


pJUMP27ts-1A KanR Type IIS Level 1 vector. Thermosensitive origin pSC101 (low copy number)

pJUMP27ts-1A KanR Type IIS Level 1 vector. Thermosensitive origin pSC101 (low copy number)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3019
    Illegal suffix found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3019
    Illegal SpeI site found at 13
    Illegal PstI site found at 27
    Illegal NotI site found at 20
    Illegal NotI site found at 3025
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3019
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3019
    Illegal suffix found at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3019
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3034
    Illegal SpeI site found at 13
    Illegal PstI site found at 27
    Illegal NgoMIV site found at 1386
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/jump/level1
//plasmidbackbone/assembly/typeiis
Parameters
resistancekanamycin