Plasmid_Backbone

Part:BBa_J428342

Designed by: Marcos Valenzuela-Ortega   Group: 2021_Engineering_Committee   (2022-06-15)


pJUMP29-1B KanR Type IIS Level 1 vector for B modules

OriV 9 (pBBR322/ROP; medium copy number)


JUMP Collection

Joint Universal Modular Plasmids (JUMP) is a modular vector platform for Golden Gate (PhytoBrick) and Biobrick assembly based on Standard European Vector Architecture (SEVA).

For more information please see: Marcos Valenzuela-Ortega, Christopher French, Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology, Synthetic Biology, Volume 6, Issue 1, 2021, ysab003, https://doi.org/10.1093/synbio/ysab003

Plasmid Name Origin (Copy) ABr Level Module Selection 2022 Distribution Location
BBa_J428341 pJUMP29-1A pBBR322 (Medium) Kanamycin Level 1 A BBa_J428326 Kit Plate 1 Well 2A
BBa_J428342 pJUMP29-1B pBBR322 (Medium) Kanamycin Level 1 B BBa_J428326 Kit Plate 1 Well 2C
BBa_J428343 pJUMP29-1C pBBR322 (Medium) Kanamycin Level 1 C BBa_J428326 Kit Plate 1 Well 2E
BBa_J428344 pJUMP29-1D pBBR322 (Medium) Kanamycin Level 1 D BBa_J428326 Kit Plate 1 Well 2G


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2944
    Illegal suffix found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2944
    Illegal SpeI site found at 13
    Illegal PstI site found at 27
    Illegal NotI site found at 20
    Illegal NotI site found at 2950
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2944
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2944
    Illegal suffix found at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2944
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2959
    Illegal SpeI site found at 13
    Illegal PstI site found at 27
    Illegal NgoMIV site found at 1386
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2184


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/jump/level1
//plasmidbackbone/assembly/typeiis
Parameters
originpBBR322
resistancekanamycin