Lpp-OmpA(46-159)-Streptavidin wild-type + His6
Assembled construct of signal peptide of lipoprotein, five transmembrane domains of outer membrane protein A, and streptavidin, wild-type with His6 tag at the end.
This construct codes for a protein which displays streptavidin on the cell surface.
NOTE ABOUT THE SEQUENCE: The mixed site between parts is 'only' six base pairs, ACTAGA. There is no spacer T or G nucleotide. These spacer nucleotides have been placed in the results for "get selected sequence" as an automatic composite-parts addition for the BioBricks mixed site between assembled parts.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 485
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 527
Illegal AgeI site found at 578
- 1000COMPATIBLE WITH RFC