RNA

Part:BBa_J23007

Designed by: bryan hernandez   Group: iGEM2006_Berkeley   (2006-07-08)

[key3b]

This part has the same homology region to J01122 as J23008 except that unlike J23008, J23007 does not contain the hairpin that was derived from J01129. J23007 anneals linearly to the stem of the hairpin in J01122 with perfect base pairing and destroys the hairpin in the process revealing the RBS on J01122. When J01122 is transcribed the RNA secondary structure is such that the RBS hairpins with sequence upstream preventing the ribosome from binding and translation from occuring thereby locking up RFP further down stream. When J23007 is introduced into the cell in trans, we suspect that it will unlock the RBS for RFP as described above allowing translation to occur.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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