Plasmid_Backbone

Part:BBa_J179001

Designed by: Jara Radeck   Group: Mascher_lab   (2013-12-20)

pBS2E: Bacillus subtilis vector, lacA integration, MLS resistance

This part is an empty backbone vector for the usage in Bacillus subtilis. It integrates in the lacA locus and can be selected with erythromycin + lincomycin (MLS). It has a ampicillin resistance for cloning in E.coli.

This version still contains NgoMIV restriction sites, therefore is not RFC25-compatible. For an RFC25-compatible version please use the BGSC ECE258.


This backbone is a BioBricked version of the B. subtilis vector pAX01.

This BioBrick is part of the LMU 2012 igem project BacillusBioBrickBox

Reference: Härtl et al.



Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6237
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4581
    Illegal NotI site found at 6112
    Illegal NotI site found at 6243
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6237
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 6237
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 6237
    Plasmid lacks a suffix.
    Illegal XbaI site found at 6252
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1462
    Illegal EcoRI site found at 6237
    Illegal XbaI site found at 6252
    Illegal SpeI site found at 2
    Illegal PstI site found at 16


[edit]
Categories
//chassis/multihost
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//plasmid/chromosomalintegration
//plasmidbackbone/operation
Parameters
chassisB. subtilis + E. coli
copies
origin
resistanceA(E. coli) E(B. subtilis)