Plasmid_Backbone

Part:BBa_J176127

Designed by: Brady Laughlin   Group: Haynes_Lab   (2011-10-24)

V0120

V0120, standard high-copy cloning vector for E. coli


Usage and Biology

This high-copy vector carries RFC23 (Silver Standard) BioBrick cloning sites for modular protein assembly.



Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal XbaI site found at 116
    Illegal SpeI site found at 1825
    Illegal PstI site found at 1839
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal SpeI site found at 1825
    Illegal PstI site found at 1839
    Illegal NotI site found at 107
    Illegal NotI site found at 247
    Illegal NotI site found at 1832
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal BglII site found at 1015
    Illegal BamHI site found at 318
    Illegal BamHI site found at 1021
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 1825
    Illegal EcoRI site found at 101
    Illegal XbaI site found at 116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 101
    Illegal XbaI site found at 116
    Illegal SpeI site found at 1825
    Illegal PstI site found at 1839
    Illegal NgoMIV site found at 4648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1571
    Illegal BsaI.rc site found at 3216
    Illegal EcoRI site found at 101
    Illegal XbaI site found at 116
    Illegal SpeI site found at 1825
    Illegal PstI site found at 1839


[edit]
Categories
Parameters
n/aV0120