Intermediate

Part:BBa_I759002

Designed by: Josh Michener   Group: iGEM07_Caltech   (2007-06-27)

pTet-spacer-cro

pTet-spacer-cro

A pTet promoter (can be repressed by tet Repressor, and transcriptional activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-cro complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in. This construct is an intermediate, as the terminator sequence for the transcript has not yet been cloned it. The finished construct can produce an aTc and cis-dependent level of cro protein.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None