Regulatory
Lux
Part:BBa_I1051
Designed by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr Group: BE.109 2006 BE.109 Instructors (2003-01-31)
Lux cassette right promoter
The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.In thisversion, a binding site for cI dimer has been added: binding to this site dominantly represses transcription.
Usage and Biology
Unspecified.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//regulation/negative
//regulation/multiple
//classic/regulatory/uncategorized
//function/cellsignalling/LuxR
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//regulation/negative
//regulation/multiple
//classic/regulatory/uncategorized
//function/cellsignalling/LuxR
Twins
More...
Parameters
| negative_regulators | 1 |
| positive_regulators | 1 |
