This part codes for an autoinducer synthetase which utilizes SAM to make the quorum sensing molecule 3OH,C14:1-HSL.
3OH,C14:1-HSL can bind CinR (Part:BBa_C0077) to make a complex which can activate the Cin promoter Part:BBa_R0077.
Usage and Biology
In Rhizobium leguminosarum, cinR and cinI are forward-pointing, adjacent, and separate transcriptional elements. There is a short (<200bp) intergenic region between cinR and cinI. The cinR/intergenic/cinI structure is conserved for the cinRI locus in Rhizobium etli, and for the cerRI locus in Rhodobacter sphaeroides. The intergenic region is thought to contain a terminator for cinR, a promoter and RBS for cinI, and a dna-binding domain for cinR and its homologs, though a conserved structure has not yet been identified.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 223
- 1000COMPATIBLE WITH RFC