Coding
luxI

Part:BBa_C0061

Designed by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr   Group: Antiquity   (2003-01-31)

autoinducer synthetase for AHL

Synthesizes 3OC6HSL, which binds to LuxR.

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.


Usage and Biology

Enzyme for creating acyl-homoserine lactones from normal cell metabolites. All LuxI proteins direct the synthesis of specific acyl-HSLs and show sequence similarity.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 637
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis/ahl
//function/cellsignalling
Parameters
directionForward
ec_numnone
keggnone
proteinluxI-LVA
swissproP12747
tagLVA
uniprotP48445