Designed by: Reshma Shetty Group: NCBS (2005-11-16)
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- Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
- Taken from Lucigen's pSMART VC vector (http://www.lucigen.com/products/Cloning_Systems/CopyRight.html). See NCBS accession number AY643800. No stem loop was easily identifiable using mFOLD. According to Lucigen, this flanking terminator enabled cloning of strong promoters which failed to clone in other vectors. Note that this sequence may be somewhat longer than what is strictly necessary for transcript termination. I was conservative in my design of this part (i.e. I was generous in how much sequence I took) in the hopes of not accidentally adversely affecting termination efficiency.
A blast search reveals that part of this terminator matches bases 4840 to 4802 of Bacteriophage T3 gene 1 to gene 11 (accession number X17255). From this similarity, the stem loop was annotated in the part.