Terminator

Part:BBa_B0013

Designed by: Reshma Shetty   Group: Antiquity   (2003-01-31)

Terminator.png

biologyT7TE (+/-)
directionForward
forward_efficiency0.6[CC]
reversed_version591
reverse_efficiency-1.06[CC]

TE from coliphage T7 (+/-)

  • Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
  • But notice that the measurement indicates it is a strong promoter in the reverse direction.

Secondary Structure

File:Mfold-B0013-1.png


Measurement


Functional Parameters: Austin_UTexas

BBa_B0013 parameters

Burden Imposed by this Part:

Burden Value: 0.6 ± 0.8%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

[edit]
Categories
//direction/forward
//terminator/single
Parameters
biologyT7TE (+/-)
directionForward
forward_efficiency0.6[CC]
reversed_version591
reverse_efficiency-1.06[CC]