Coding

Part:BBa_K3657009

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)


RNA binding Pentatricopeptide Repeat Protein (Guanine)

The Pentatricopeptide Repeat Proteins (PPR) are 35 amino acid long subunits, that specifically bind one of the RNA bases. The specificity is determined by the amino acids in position 3 and 34. Multiple PPRs can be assembled into one protein.


This part is the guanine binding PPR.


It can be assembled to a RNA binding Protein when combined with the other PPRs:

Adenine:BBa_K3657006

Uracil:BBa_K3657007

Cytosine:BBa_K3657008


See also examples of the composite parts: BBa_K3657012, BBa_K3657013

Usage and Biology

PPRs are originally found in Arabidopsis thaliana and can bind RNA. In chloroplasts, they take part in many post-translational regulation processes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None