Coding

Part:BBa_K3468528

Designed by: Haoyang Shi   Group: iGEM20_TJUSLS_China   (2020-10-23)


PETase S214A

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from S to A at 214 position which can be more stable in higher temperature compared with the wild type. The DDG of this mutant is -0.3454.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 539
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 539
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 539
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 539
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None