Coding

Part:BBa_K3468379

Designed by: Xujia Liu   Group: iGEM20_TJUSLS_China   (2020-10-23)


PETase R132Q

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from R to Q at 132 position which can be more stable in higher temperature compared with the wild type.The DDG of this mutant is -0.1704.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 292
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 292
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None