Coding

Part:BBa_K3468047

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)


PETase S223P

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from S to P at 223 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This mutant undergoes proline substitution. Proline has a special ring structure, which can increase the rigidity of the loop, reduce the conformational entropy, and improve the thermal stability of the protein.

Maestro website evaluated an result of -1.214，IMUTANT website evaluated a decrease result of -0.32, and an evaluation of FoldX also obtained a thermal stability improvement result of -0.47 kcal/mol. And the result was not ideal.

Fig.1 S233P in PyMOL
Fig.1 WT S233 in PyMOL
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Categories
Parameters
None