Part:BBa_K3100020

ybaS(antiacid gene)

Usage and Biology:

YbaS is a glutaminase that mediates an acid tolerance system. This acid tolerance system in E. coli that relies on L-glutamine (Gln), one of the most abundant food-borne free amino acids. Upon uptake into E. coli, Gln is converted to L-glutamate (Glu) by the acid-activated glutaminase YbaS, with concomitant release of gaseous ammonia [1]. The free ammonia neutralizes proton, resulting in elevated intracellular pH under acidic environment. Not only that, YbaS and the amino acid antiporter GadC, which exchanges extracellular Gln with intracellular Glu, together constitute an acid resistance system that is sufficient for E. coli survival under extremely acidic environment.

Characterization:

We have added quantitative experimental characterization data to an existing Part BBa_ from the Registry of Standard Biological Parts. More imformation please click here:https://parts.igem.org/Part:BBa_K1675002

References:

[1]Lu P, Ma D, Chen Y, et al. L-glutamine provides acid resistance for Escherichia coli through enzymatic release of ammonia. Cell Res. 2013;23(5):635-44.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]