Coding

Part:BBa_K3054016

Designed by: Dharmik Hitesh Patel   Group: iGEM19_Chalmers-Gothenburg   (2019-10-20)


bphA2 protein from Rhodococcus jostii + Flag tag

Rhodococcus jostii was found to degrade lightly chlorinated PCB via a mutated version of biphenyl pathway. Codon optimized to express in yeast.



Usage and Biology

IGEM pathway.jpeg

Us, iGEM Chalmers Gothenburg, planned to engineer the following pathway to degrade a large spectrum of PCBs. A detailed pathway is shown in the diagram. This biobrick is a component of this pathway optimized to be expressed in yeast.


BphA protein

BphA is a multicomponent biphenyl dioxygenase system is composed of a ferredoxin reductase (BphA4), a ferredoxin (BphA3) and a terminal oxygenase (BphA1A2)

BphA2: Biphenyl 2,3-dioxygenase subunit beta


Source: UniProtKB - Q53123 (BPHA2_RHOJR)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None