Composite

Part:BBa_K3003043

Designed by: Ömer Can Ergül   Group: iGEM19_Bilkent-UNAMBG   (2019-10-21)

ProD sfGFP Ag43

This part is created by the Ag43 cassette with sfGFP. Ag43 displays the sfGFP on the cell surface. There is a TEV protease cleavage site between sfGFP and the Ag43 autotransporter allowing the cleavage of the fluorescent protein with the TEV protease.We measured the fluorescence in the supernatant that the cleaved sfGFP molecules generated. The RFU/OD600 graphs can be seen below, these graphs contain data from 2 different strains.

800px-T--Bilkent-UNAMBG--pzssfgfp.jpg.png

800px-T--Bilkent-UNAMBG--pzasfgfp.jpg.png

800px-T--Bilkent-UNAMBG--yooooooooooo.jpg.png

800px-T--Bilkent-UNAMBG--pzssfgfpod.jpg.png

800px-T--Bilkent-UNAMBG--pzasfgfpod.jpg.png

800px-T--Bilkent-UNAMBG--jalkfsjaklsfjaslkf.jpg.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1282
    Illegal PstI site found at 1840
    Illegal PstI site found at 2440
    Illegal PstI site found at 3064
    Illegal PstI site found at 3335
    Illegal PstI site found at 3388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1282
    Illegal PstI site found at 1840
    Illegal PstI site found at 2440
    Illegal PstI site found at 3064
    Illegal PstI site found at 3335
    Illegal PstI site found at 3388
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 973
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1282
    Illegal PstI site found at 1840
    Illegal PstI site found at 2440
    Illegal PstI site found at 3064
    Illegal PstI site found at 3335
    Illegal PstI site found at 3388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1282
    Illegal PstI site found at 1840
    Illegal PstI site found at 2440
    Illegal PstI site found at 3064
    Illegal PstI site found at 3335
    Illegal PstI site found at 3388
    Illegal NgoMIV site found at 216
    Illegal NgoMIV site found at 1108
    Illegal NgoMIV site found at 2332
    Illegal NgoMIV site found at 2842
    Illegal NgoMIV site found at 2863
    Illegal AgeI site found at 1858
    Illegal AgeI site found at 2602
    Illegal AgeI site found at 3016
    Illegal AgeI site found at 3258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI.rc site found at 262


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Categories
Parameters
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