DNA

Part:BBa_K2927031

Designed by: KUO, PEI-CHING   Group: iGEM19_CCU_Taiwan   (2019-09-12)


Positive control sequence for crRNAs target

The positive control vector is generated for confirmation of the Cas12a-crRNA activity without using African swine fever virus (ASFV) genome. A fragment containing target sequences of six crRNAs are cloned and amplified by yTA vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None