DNA

Part:BBa_K2644302

Designed by: Junjing Chen   Group: iGEM18_TJU_China   (2018-09-29)


sJanus+linker.b

SOD2 is a mitochondrial-target sequence, allowing CRISPR Cas9 protein complex locating into mitochondrion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 187
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 196


Usage

SOD2-MTS is an essential part in our group. It is mitochondrial targeting signal derived from SOD2, a 34 amino acid long peptide that directs a synthesized Cas9 protein to the mitochondria. It is found at the N-terminus end consists of an alternating pattern of hydrophobic and positively charged amino acids to form what is called an amphipathic helix. SOD2 can contain additional signals that subsequently target the protein to different regions of the mitochondria, such as the mitochondrial matrix.

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