Composite
ChiB4A

Part:BBa_K2594013

Designed by: Yazmin Camacho   Group: iGEM18_US_AFRL_CarrollHS   (2018-10-10)


Chitinase B4A

This part is engineered to express chitinase originated from Serratia marcescens B4A fused to the N- and C-term domains of Pseudomonas syringae ice-nucleation protein. The fusion protein will be anchored to the Escherichia coli cell surface. Chitinase is an enzyme that degrades chitin, a major component in the structure of cell walls for plant and fungi cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1045
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 551
    Illegal NgoMIV site found at 2443
    Illegal AgeI site found at 405
    Illegal AgeI site found at 2146
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 580
    Illegal SapI site found at 1402
    Illegal SapI.rc site found at 238


[edit]
Categories
Parameters
None