Part:BBa_K2572035
Tcl42-mWasabi
This part is a composite of Tcl42 and the fluorescent reporter mWasabi. The former is an upshifted variant of a well-known temperature-sensitive variant of the bacteriophage λ repressor cI (mutant cI857, here referred to as TcI) acting on a tandem pR–pL operator–promoter. The potential utility of these devices in three distinct in vivo scenarios relevant to mammalian microbial therapy is now well demonstrated. These scenarios include spatially selective activation within a mammalian host using focused ultrasound, sensing and response to a fever, and self-destruction at ambient temperatures to prevent environmental contamination after leaving the intended host. This coincides largely with our team’s work. The inducing temperature, 42 degrees Celsius, is our designed threshold for bacterial suicide.
The part was characterized by us as effective and no leakage at 36 degrees Celsius was detected. However, we did find some spill expression at 39 degrees Celsius, so the part need to be further refined to come to practical use in our project.
quantitiative data needed
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 388
Illegal BsaI site found at 799
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