Regulatory

Part:BBa_K2569023

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-09-29)

Pfat, constitutive promoter in Pseudomonas. fluorescence pf-5

Promoter Pfat is taken from the upstream region of fat acid, whose length is 70bp. It is one of the constitutive promoters of Pseudomonas. fluorescence promoter library, its strength is characterized by a reporter gene, firefly luciferase. It can be used to regulate the gene expression in Pseudomonadaceae through 3A Assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None