Coding

Part:BBa_K2510101

Designed by: Aya Gomaa   Group: iGEM17_Paris_Bettencourt   (2017-10-27)


PDDronpa wt t7 promoter

This part is the same exact dronpa version as the part Part:BBa K2510100, except that it has T7 promoter and lac operator.

Dronpa is a reversible photoswitchable fluorescent protein that is switched on by default “fluorescent” and is switched off when illuminated by cyan light (~500nm). Dronpa Fluorescence is recovered by shining violet light (~400nm). And has been used in a design that facilitates the optical control of protein activities This part contains a device of two copies of Dronpa Fluorescent Protein that are codon optimized for E Coli with two BsaI cutting site in between to allow the insertion of various proteins to be tested </p? Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 805
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 808
    Illegal BsaI.rc site found at 802


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