Regulatory

Part:BBa_K2510029

Designed by: Alma Chapet--Batlle   Group: iGEM17_Paris_Bettencourt   (2017-10-27)


O-TetR - T7 - O-TetR - O-P22C2 promoter



Cell-free testing

This synthetic operator was part of the library that we used to test the activity of repressors caged with Dronpa

<img src="96_conditions.png"> 

                            Figure 1 :  the experiment conducted with the repressors caged with Dronpa
                                        
                                   </span>

                                     <img src="Repression_strength_of_closed_state.png ">

                                      Figure 2 :  An over view of the results of the cell-free experiment. Each 
                                       promoter was tested with its cognate repressors. Our results show that in 90% of our 
                                       constructs caging the repressors with Dronpa has increased the repression strength. 
                                       We have also obtained a wide linear range of repression strength-indicated by the red 
                                       slope-.
                                   </span>
                                   <img src="TetR_dronpa.png">
                                   <img src="P22c2_dronpa.png">
                                    <img src="HKCI_caged_dronpa.png"> 
                                   
                                           Figure 3: detailed Results of the cell-free experiment. Each promoter was 
                                           tested with its cognate repressors. Top: Testing with TetR caged with 
                                           either wt-Dronpa (BBa_K2510108) or a mutated version(BBa_K2510109)Middle: 
                                           Testing with P22c2 caged with either wt-Dronpa (BBa_K2510112) or a mutated 
                                            version(BBa_K2510113).Bottom: Testing with HKcI caged with either wt-
                                           Dronpa (BBa_K2510110) or a mutated version(BBa_K2510111)           

                                  <img src="Wtvs_mut_repressors.png" >
                                   Figure 4: the wtDronpa has a better control of HKC1 and C2P22 repressors while the mutDronpa has a better control of TetR repressors </span>


qdkfjgs@

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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