Part:BBa_K2408002

4CL

4CL (4-coumarate--CoA ligase 2) from Vitis vinifera optimized for S. cerevisae.

4-coumarate--CoA ligase 1 is an ATP dependent enzyme that catalyzes the conversion of p-coumaric acid to p-Coumaroyl-CoA. It is the second enzyme in the resveratrol biosynthesis pathway.
Enzyme activity: ATP + p-coumaric acid + CoA => AMP + diphosphate + p-Coumaroyl-CoA
This sequence contains ‘6 His tag’ downstream from the gene, and was optimised for S.cerevisiae using IDT sequence optimisation tool.

Hamberger, B., & Hahlbrock, K. (2004). The 4-coumarate: CoA ligase gene family in Arabidopsis thaliana comprises one rare, sinapate-activating and three commonly occurring isoenzymes. Proceedings of the National Academy of Sciences, 101(7), 2209-2214.‏ ISO 690

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 424
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1531
  Illegal BamHI site found at 1150
  Illegal BamHI site found at 1194
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]