Coding

Part:BBa_K2372006

Designed by: Marcel Chris Conrady   Group: iGEM17_Tuebingen   (2017-10-26)


GyrBR from E.coli

Gyrase B (GyrB) is a type II topoisomerase that usually occurs in Escherichia coli. It binds to DNA and keeps chromosomes in an unwound state by negatively supercoiling closed circular double-stranded DNA in an ATP-dependent manner. DNA Gyrase subunit B (GyrB) is the target of aminocoumarin antibiotics. GyrBR is a resistant variant. If expressed in E.coli it should be able to form a heterodimer with DNA Gyrase subunit A (GyrA). It can be used to show that the target of a new antibiotic is the ATP binding site in GyrB.

GyrB (Ecoli
Function relaxes positive supercoiled DNA during transcription and amplification
Use in Prokaryotic cells
RFC standard RFC 10
Backbone pSB1C3
Organism E.coli K12/LE234
Source IDT (DNA synthesis)
Submitted by Tuebingen 2017



References:
http://www.uniprot.org/uniprot/P0AES6


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 238
    Illegal AgeI site found at 931
    Illegal AgeI site found at 2047
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None