Translational_Unit

Part:BBa_K2182002

Designed by: David Rosenberg, Joshua Timmons   Group: iGEM16_Northeastern   (2016-10-14)


Expression for Proteorhodopsin and BLH

This part generates proteorhodopsin, a membrane-bound proton pump, and Blh, an enzyme which produces all-trans retinal from beta-carotene. All-trans retinal is a necessary co-factor for proteorhodopsin function and as such this part contains the whole functional system to move protons when the culture is supplied with beta-carotene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1438
    Illegal XhoI site found at 1011
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 245
    Illegal AgeI site found at 1200
    Illegal AgeI site found at 1575
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None