Coding

Part:BBa_K1473009

Designed by: Shan, Zhe   Group: iGEM14_TCU_Taiwan   (2014-09-26)


pRha induced CRISPR

Here we combined a CRISPR system(BBa_K1218011) with an inducible promoter(BBa_K914003). The CRISPR system contains a tracrRNA , a Cas9 protein and a minimal CRISPR array, it functions to knockout target gene. We give this system an inducible promoter so we can decided when to switch if on or off.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1570
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3849
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4794
    Illegal BsaI.rc site found at 4768


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Parameters
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