Coding

Part:BBa_K1396000

Designed by: Ariel Hernandez-Leyva   Group: iGEM14_Yale   (2014-10-10)


pSB1C3: 2XStrep_Flagtag_LL-37_Mussel Foot Protein_sfGFP

The part is an coding sequence for an anti-microbial peptides linked to a mussel-foot protein-linked to superfolder GFP for localization. The mussel foot protein will anneal to surfaces as a wet glue and the antimicrobial domain is designed to interact with microbial membranes and interfere with membrane stability. In order to use this part you can produce it in a TAG recoded organism simultaneously expressing a Tyrosine supressor or L-DOPA orthogonal translational system. In order to purify you can use the 2X Strep tag and strep column and later cleave with enterokinase to remove the sequence supressing LL-37 antimicrobial action.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 985
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 232
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1634


[edit]
Categories
//collections/antimicrobial
Parameters
None