Protein_Domain

Part:BBa_K1092105

Designed by:   Group: iGEM13_Hong_Kong_CUHK   (2013-09-18)

RFP N terminus

An RFP fragment adapted from the RFP part BBa_E1010, and its counterpart is the RFP C terminus (BBa_K1092106). It is adapted to be expressed downstream of a fusion protein such as our Voltage switch parts (BBa_K1092023 & BBa_K1092024). It is the N terminal of the RFP, and is capable of refolding with it’s counter part C terminal as demonstrated by the NYMU-Taipei 2010 iGEM team through the Biomolecular Fluorescent complementation (BiFC) to give fluorescent (BBa_K411103 & BBa_K411104). We adapt this protein to be the downstream of our voltage switch because we want it to become our effector of the switch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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