Coding

Part:BBa_K1072001

Designed by: Yichong wang, Zhiwei li, Xihao liao and Junjie zhang   Group: iGEM13_SCUT   (2013-09-01)

NADH Dehydrogenase Coding Region

BBa_K1072001 is the coding region of NADH dehydrogenase which is an enzyme following chemical reaction:

NADH + H+ + acceptor ↔ NAD+ + reduced acceptor

The ndh gene can express enzyme NDH-2 to generate H2O2 vapor which is an additional activator of the luxI promoter in oscillator system by changing cells redox state transiently. H2O2 vapor can migrate between colonies and then synchronize them.


We transformed the BBa_K1072002 into the cells and detected highter H2O2 concentration than blank.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
proteinNADH Dehydrogenase