Composite

Part:BBa_K1065303

Designed by: Fabio Digiacomo   Group: iGEM13_UNITN-Trento   (2013-08-13)

B0034-YF1-B0034-FixJ-pFixK2


This is an intermediate part to build a blue light inducible genetic circuit. The part consists of the coding sequences of the blue light sensor YF1, its Response Regulator (FixJ) and the inducible promoter controlled by the RR (pFixK2). In the dark, YF1 autophosphorylates and than transfers the phosphate to FixJ. Phosphorilated FixJ activates FixK2 promoter. The part presents RBS-coupled YF1 and FixJ but it doesn't present any terminator. BBa_K2277555 is the new part which has been improved on the basis of this part.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 634
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 1641
    Illegal AgeI site found at 276
    Illegal AgeI site found at 1422
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1311
    Illegal BsaI.rc site found at 175


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Categories
Parameters
None