Cell
TOP10

Part:BBa_V1009

Designed by: Randy Rettberg   Group: Arkin Lab   (2005-07-14)

TOP10

Chemically competent E.coli from [www.invitrogen.com Invitrogen].

Usage and Biology

  • Very similar to DH10B
  • an MC1061 derivative (Casadaban80). Prepare cells for chemical transformation with CCMB80 buffer Here
  • Streptomycin resistant


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
genotypeF- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1
araD139 Δ(ara-leu)