TetR repressible promoter
Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc.
Usage and Biology
Medium strength promoter. [jb, 5/24/04]From the reference article:
"In contrast to tetracycline, anhydrotetracycline is a particularly useful inducer. It binds Tet R with an ~35-fold higher binding constant and thus allows to operate at very low concentrations. At the same time, its antibiotic activity is ~100-fold lower and concentrations of <50 ng/ml as required for the full induction of P LtetO-1 have no effect on the growth of E.coli."
Improved by Fudan-CHINA in 2018
This year, Fudan-CHINA makes a mutation to Part:BBa_R0040 to improve its combination with the tTA transcriptional factor by computational simulation.Please be free to visit Part:BBa_K2886010.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC