Plasmid_Backbone

Part:BBa_K801004

Designed by: Ingmar Polte   Group: iGEM12_TU_Munich   (2012-09-21)

pTUM104 yeast shuttle vector with GAL1 promoter

This part is based on the plasmid pTUM100 (BBa_K801000). The galactose-inducible GAL1 yeast promoter has been cloned in front of the MCS (RFC25 prefix and suffix).

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 5799
    Illegal suffix found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5799
    Illegal NheI site found at 3275
    Illegal SpeI site found at 11
    Illegal PstI site found at 25
    Illegal NotI site found at 18
    Illegal NotI site found at 5805
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5799
    Illegal XhoI site found at 5826
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5799
    Illegal suffix found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5799
    Illegal XbaI site found at 5814
    Illegal NgoMIV site found at 5832
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1432
    Illegal BsaI.rc site found at 2378
    Illegal EcoRI site found at 5799
    Illegal XbaI site found at 5814
    Illegal SpeI site found at 11
    Illegal PstI site found at 25


[edit]
Categories
//chassis/eukaryote/yeast
//chassis/prokaryote/ecoli
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/inducible
Parameters
None