Plasmid_Backbone

Part:BBa_K801003

Designed by: Georg Schützinger   Group: iGEM12_TU_Munich   (2012-09-21)

pTUM103 yeast shuttle vector with pADH1 promoter

This part corresponds to pTUM100 (BBa_K801000). The constitutive pADH1 promoter has been cloned into the MCS (RFC25 prefix and suffix). Part is compatible with RFC 10 and RFC 25

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 776
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 4041
    Illegal SpeI site found at 777
    Illegal PstI site found at 791
    Illegal NotI site found at 42
    Illegal NotI site found at 784
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 777
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Plasmid lacks a suffix.
    Illegal XbaI site found at 51
    Illegal SpeI site found at 777
    Illegal PstI site found at 791
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 149
    Illegal BsaI.rc site found at 2198
    Illegal BsaI.rc site found at 3144
    Illegal EcoRI site found at 36
    Illegal XbaI site found at 51
    Illegal SpeI site found at 777
    Illegal PstI site found at 791


[edit]
Categories
//chassis/eukaryote/yeast
//chassis/prokaryote/ecoli
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/constitutive
Parameters
None