Plasmid_Backbone

Part:BBa_K801002

Designed by: Georg Schützinger   Group: iGEM12_TU_Munich   (2012-09-21)

pTUM102 yeast shuttle vector with pTEF2 promoter

This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF2 promoter has been into the MCS (RFC25 prefix and suffix). Part is compatible with RFC 10

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 705
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3970
    Illegal SpeI site found at 706
    Illegal PstI site found at 720
    Illegal NotI site found at 42
    Illegal NotI site found at 713
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 706
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Plasmid lacks a suffix.
    Illegal XbaI site found at 51
    Illegal SpeI site found at 706
    Illegal PstI site found at 720
    Illegal NgoMIV site found at 194
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2127
    Illegal BsaI.rc site found at 3073
    Illegal EcoRI site found at 36
    Illegal XbaI site found at 51
    Illegal SpeI site found at 706
    Illegal PstI site found at 720


[edit]
Categories
//chassis/eukaryote/yeast
//chassis/prokaryote/ecoli
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/constitutive
Parameters
None