Plasmid_Backbone

Part:BBa_K801001

Designed by: Georg Schützinger   Group: iGEM12_TU_Munich   (2012-09-21)

pTUM101 yeast shuttle vector with pTEF1 promoter

This part corresponds to pTUM100 (BBa_K801000). The constitutive pTEF1 promoter has been cloned into the MCS (RFC25 prefix and suffix).

Vector is compatible with RFC 10 and RFC 25

Sequence and Features

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3771
    Illegal SpeI site found at 507
    Illegal PstI site found at 521
    Illegal NotI site found at 42
    Illegal NotI site found at 514
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Plasmid lacks a suffix.
    Illegal XbaI site found at 51
    Illegal SpeI site found at 507
    Illegal PstI site found at 521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 261
    Illegal BsaI.rc site found at 1928
    Illegal BsaI.rc site found at 2874
    Illegal EcoRI site found at 36
    Illegal XbaI site found at 51
    Illegal SpeI site found at 507
    Illegal PstI site found at 521


[edit]
Categories
//chassis/eukaryote/yeast
//chassis/prokaryote/ecoli
//plasmidbackbone/copynumber/high
//plasmidbackbone/expression/constitutive
Parameters
None