Other
TAT-trojan

Part:BBa_K792003

Designed by: Manuel Giménez   Group: iGEM12_Buenos_Aires   (2012-09-24)

Import enhancer - HIV TAT penetratin (trojan peptide)

Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Jones et al 2005]. They can be used to increase the efficiency with which a protein enters a cell.


This part is the penetratin from the HIV TAT protein, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.


This part includes a HindIII restriction site as an extra.

See also: Another Trojan Peptide part BBa_K792004
BsAs2012-Biohazard.png Trojan peptides can penetrate cell membranes. Extreme your safety measures when dealing with strains expressing this part: avoid direct contact with them or conditioned medium and discard them as pathogenic residues.
This parts was originally built to be used in iGEM BsAs 2012 project to implement a cross-feeding regulatory system between two different yeast strains with specific auxotrophy. Visit our wiki to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part. Igem.bsas.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//proteindomain/internal/special
Parameters
None